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Registervariation in response of the analyte and internal standard [Bioanalytics]
Dear Helmut,
Thanks for replying me
I don't have any problem about getting the extracts into solution, I use diethylether:n-hexane (7:3) mixture during extraction, after evaporation step of my procedure; reconstitution was done mobile phase before but result were not satisfying so water+25mM ammonia+ACN (50:50), pH: 9.9 mixture is begin to use.
Also; validation was completed and this problem is occured in study so, I cannot change any of my conditions/solutions/pH.
By the way; I think this problem leads to ionization problem. What can you think about changing desolvation temperature and desolvation gas flow? And if I change desolvation temperature and/or desolvation gas flow, do I have to perform whole validation once more?
Edit: Full quote removed. Please delete anything from the text of the original poster which is not necessary in understanding your answer; see also this post! [Helmut]
Thanks for replying me
I don't have any problem about getting the extracts into solution, I use diethylether:n-hexane (7:3) mixture during extraction, after evaporation step of my procedure; reconstitution was done mobile phase before but result were not satisfying so water+25mM ammonia+ACN (50:50), pH: 9.9 mixture is begin to use.
Also; validation was completed and this problem is occured in study so, I cannot change any of my conditions/solutions/pH.
By the way; I think this problem leads to ionization problem. What can you think about changing desolvation temperature and desolvation gas flow? And if I change desolvation temperature and/or desolvation gas flow, do I have to perform whole validation once more?
Edit: Full quote removed. Please delete anything from the text of the original poster which is not necessary in understanding your answer; see also this post! [Helmut]
Complete thread:
- variation in response of the analyte and internal standard haydonat 2011-01-13 10:27
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- variation in response of the analyte and internal standard Helmut 2011-01-13 17:08
- variation in response of the analyte and internal standardhaydonat 2011-01-15 15:16
- variation in response of the analyte and internal standard Ohlbe 2011-01-15 23:09
- variation in response of the analyte and internal standardhaydonat 2011-01-15 15:16
- variation in response of the analyte and internal standard Ohlbe 2011-01-13 19:14
- variation in response of the analyte and internal standard haydonat 2011-01-15 15:22
- variation in response of the analyte and internal standard NewInPK 2011-02-01 18:32
- variation in response of the analyte and internal standard Ohlbe 2011-02-02 10:22
- variation in response of the analyte and internal standard Helmut 2011-01-13 17:08
Ing. Helmut Schütz