Bioequivalence and Bioavailability Forum

Dear Vajra,

❝ When integrating a batch whether i have to use only integration parameter for the IS in the analyte A and analyte B or I can apply separate integration parameters for the IS in analyte A and analyte B respectively.

You can have different integration parameters for each peak (A, B and IS), and optimise them so that you get a correct integration for each. But why would you want to use different integration parameters for IS when quantifying A and B ? Either your IS peak is correctly integrated and there is no reason to repeat the work and re-integrate differently, or it is not correctly integrated and you need to change for both. Using separate integration parameters may arise suspicion that you are doing this to tweak the data to get the results you want, or to make failing runs pass.

Regards
Ohlbe