Bioequivalence and Bioavailability Forum • Recovery vs. overall efficiency

Recovery vs. overall efficiency [Bioanalytics]

posted by drajasekhar – India, 2009-12-30 19:17 (6017 d 04:53 ago) – Posting: # 4541
Views: 6,801

Dear Ohlbe,

Thank you very much for your clarification. Please tell me the overall efficiency and absolute recovery are the same or not!

And now, you please give me a conclusion for the following comparison i.e. Overall efficiency Vs Limit of Quantitation (here I included the LOQ and the instrumentation):


                       CASE I.

Extraction technique : Protein Precipitation Technique with Acetonitrile.

Internal Standard    : Only One Internal Standard was used

                       for both the polarities.

Overall efficiencies : Drug A; LQC: 101%, MQC: 95%, HQC: 90%

                       Drug B; LQC: 36%, MQC: 37%, HQC: 35%

Limit of Quantitation: 0.1 ng/mL for and 1.0 ng/mL

Instrumentation      : LC-MS/MS; API 5000.



                       CASE II.

Extraction technique : Liquid-liquid Extraction Technique with tBME

Internal Standard    : Two Internal Standards (one for +ve ion mode and

                       other for -ve ion mode) were used.

Overall efficiencies : Drug A; LQC: 66%, MQC: 71%, HQC: 72%

                       Drug B; LQC: 71%, MQC: 71%, HQC: 68%

Limit of Quantitation: 1.0 ng/mL for both the drugs

Instrumentation      : LC-MS/MS; API 4000.

With regards,

Rajasekhar.

Edit 1: Removed details of the method which were already given in a previous post.
Learn how to format your posts and use the Preview - you would have noticed the mess immediately! Quote from the Instructions:

Please do not include [...] series of blanks, or tabs in your text. [...] series of blanks and/or tabs will be stripped and cut down on a single blank. Both multiple blanks and tabs may look nice on your system, but awful on others.

I'm not in the mood of removing your combinations of multiple blanks and tabs all the time! [Helmut]
Edit 2: Since you reverted my first edit to your original version, I must assume that you want to start an edit-war. This is the first warning. Adhere to the Forum's Policy or your account will be blocked. [Helmut]

Complete thread:

IS (polarity switching mode) drajasekhar 2009-12-27 10:43
- IS (polarity switching mode) Ohlbe 2009-12-28 01:00
  - LC-MS/MS method in the ionization Switching modes drajasekhar 2009-12-28 06:32
    - Recovery? Ohlbe 2009-12-30 13:51
      - Recovery? drajasekhar 2009-12-30 14:45
        
        Recovery vs. overall efficiency Ohlbe 2009-12-30 17:36
        
        Recovery vs. overall efficiencydrajasekhar 2009-12-30 18:17
        
        Recovery vs. overall efficiency Ohlbe 2009-12-30 19:16
        Recovery vs. overall efficiency drajasekhar 2009-12-30 19:50
        Recovery vs. overall efficiency Helmut 2009-12-30 19:58
        
        Recovery vs. overall efficiency drajasekhar 2009-12-30 20:35
        
        Recovery vs. overall efficiency Helmut 2009-12-30 20:38
        Recovery vs. overall efficiency Ohlbe 2009-12-30 20:43
        
        Recovery vs. overall efficiency drajasekhar 2009-12-30 20:54
  - Matrix Effect drajasekhar 2009-12-28 07:00
    - Matrix Effect Helmut 2009-12-28 14:18
    - Matrix Effect Ohlbe 2009-12-30 13:53