Bioequivalence and Bioavailability Forum • LC-MS/MS method in the ionization Switching modes

LC-MS/MS method in the ionization Switching modes [Bioanalytics]

posted by drajasekhar – India, 2009-12-28 07:32 (6028 d 00:39 ago) – Posting: # 4530
Views: 7,086

Dear Members,

Please give me a conclusion for the following:

Drug A (Positive ion mode) Drug B (Negative ion mode)


                       CASE I.

Extraction technique : Protein Precipitation Technique with Acetonitrile.

Column               : C8 column

Mobile Phase         : 1 mM Ammonium acetate solution:

                       formic acid:

                       methanol and acetonitrile

                       [65:17.5:17.5, v/v/v (%)].

Internal Standard    : Only One Internal Standard was used

                       for both the polarities.

Extraction Recoveries: Drug A; LQC: 101%, MQC: 95%, HQC: 90%

                       Drug B; LQC: 36%, MQC: 37%, HQC: 35% 

Analytical Run Time  : 6.0 min



                       CASE II.

Extraction technique : Liquid-liquid Extraction Technique with tBME

Column Used          : C18 column.

Mobile Phase used    : Acetonitrile: 10 mM ammonium acetate buffer

                       [85:15,v/v(%)]

Internal Standard    : Two Internal Standards (one for +ve ion mode and

                       other for -ve ion mode) were used.

Extraction Recoveries: Drug A; LQC: 66%, MQC: 71%, HQC: 72%

                       Drug B; LQC: 71%, MQC: 71%, HQC: 68% 

Analytical Run Time  : 4.0 min.

Thanks in Advance,

Rajasekhar.

Edit: Your post was a mess! See here for Instructions. Please use the Preview before submitting your post. Don't open new threads which essentially deal with the same (your) problem. I linked your post to the old thread. [Helmut]

Complete thread:

IS (polarity switching mode) drajasekhar 2009-12-27 10:43
- IS (polarity switching mode) Ohlbe 2009-12-28 01:00
  - LC-MS/MS method in the ionization Switching modesdrajasekhar 2009-12-28 06:32
    - Recovery? Ohlbe 2009-12-30 13:51
      - Recovery? drajasekhar 2009-12-30 14:45
        
        Recovery vs. overall efficiency Ohlbe 2009-12-30 17:36
        
        Recovery vs. overall efficiency drajasekhar 2009-12-30 18:17
        
        Recovery vs. overall efficiency Ohlbe 2009-12-30 19:16
        Recovery vs. overall efficiency drajasekhar 2009-12-30 19:50
        Recovery vs. overall efficiency Helmut 2009-12-30 19:58
        
        Recovery vs. overall efficiency drajasekhar 2009-12-30 20:35
        
        Recovery vs. overall efficiency Helmut 2009-12-30 20:38
        Recovery vs. overall efficiency Ohlbe 2009-12-30 20:43
        
        Recovery vs. overall efficiency drajasekhar 2009-12-30 20:54
  - Matrix Effect drajasekhar 2009-12-28 07:00
    - Matrix Effect Helmut 2009-12-28 14:18
    - Matrix Effect Ohlbe 2009-12-30 13:53