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Registercentrifugation [Bioanalytics]
Dear Manish
Goog morning
After centrifugation, the blood separates into an upper plasma fraction, comprising ~60% of the sample volume, a lower red blood cell fraction comprising ~40%, and a thin interface layer, called the "buffy coat". Mostly this is done at RPM 3000 for 10-15 min.
And if the samples are centrifuged for time less than the specified time, the ratio of plasma and the cells will differ and chances of getting much fraction of plasma gets reduced. In a BA/BE study it will surely effect the plasma volume, as our interest would the amount of drug present in plasma.
And to know more it would be better for you to document it as protocol deviation, so that further investigation can be made.
Regards
DIXIT
Goog morning
After centrifugation, the blood separates into an upper plasma fraction, comprising ~60% of the sample volume, a lower red blood cell fraction comprising ~40%, and a thin interface layer, called the "buffy coat". Mostly this is done at RPM 3000 for 10-15 min.
And if the samples are centrifuged for time less than the specified time, the ratio of plasma and the cells will differ and chances of getting much fraction of plasma gets reduced. In a BA/BE study it will surely effect the plasma volume, as our interest would the amount of drug present in plasma.
And to know more it would be better for you to document it as protocol deviation, so that further investigation can be made.
Regards
DIXIT
Complete thread:
- centrifugation manish_ranbaxy 2009-03-24 17:26
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- centrifugation babun14 2009-03-26 10:26
- centrifugation manish_ranbaxy 2009-03-29 13:29
- centrifugationdixit 2009-03-30 06:30
- centrifugation manish_ranbaxy 2009-03-30 10:55
- concentration vs. amount Helmut 2009-03-30 13:20
- concentration vs. amount dixit 2009-03-31 12:28
- centrifugationdixit 2009-03-30 06:30
Ing. Helmut Schütz