analyte stabilisation [Bioanalytics]

posted by Helmut Homepage – Vienna, Austria, 2009-02-16 15:39 (6341 d 22:20 ago) – Posting: # 3250
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Dear Dixit!

❝ For some drugs after the blood samples are centrifuged stabilising buffers are being added to plasma samples.


… and for some drugs even immediately after blood harvesting (before centrifugation).

❝ So I would like to the criteria for buffer selection.


The one preventing degradation. ;-)
Seriously: Check the literature… instead of recreating the wheel. Examples are AgNO3 for ISDN, H3PO4 for ASA, CH3COOH for cefaclor, citrate for erythromycin, N-ethylmaleimide for captopril,…

Anyhow: You have to check the suitability of the stabiliser during development of the analytical method (haemolysis, chromatographic interference, matrix effect,…)
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