Calculation of Stock solution [Bioanalytics]
Dear all,
If we got the monohydrated version of the drug ex. Doxycycline Monohydrate and we have to prepare stock solution of Doxycycline 1000 micrograms/ml. according to COA we got the assay as is basis for the monohydrated version. Then how we have to proceed for the calculation?
The formula is weigh/volume*assay*molecular weight of analyte/mol.weight of drug*10=1000 mic gram/ml
In the above case we have to take mol.weigh of monohydrate divided by mol.weight of analyte? or both monohydrated versions? we are quantifying only the doxycycline not doxycycline monohydrate.
If we got the monohydrated version of the drug ex. Doxycycline Monohydrate and we have to prepare stock solution of Doxycycline 1000 micrograms/ml. according to COA we got the assay as is basis for the monohydrated version. Then how we have to proceed for the calculation?
The formula is weigh/volume*assay*molecular weight of analyte/mol.weight of drug*10=1000 mic gram/ml
In the above case we have to take mol.weigh of monohydrate divided by mol.weight of analyte? or both monohydrated versions? we are quantifying only the doxycycline not doxycycline monohydrate.
Complete thread:
- Calculation of Stock solutionnavin 2008-11-21 09:15
- Calculation of Stock solution vpardhasaradhi 2008-11-22 14:23
- Calculation of Stock solution Debbie 2009-07-22 18:21
- Calculation of Stock solution Ohlbe 2009-07-22 23:06
- Calculation of Stock solution Debbie 2009-07-22 18:21
- Calculation of Stock solution vpardhasaradhi 2008-11-22 14:23
