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Dear Alhas,
You can't calculate a concentration in a sample without IS, by comparison to a standard curve with IS ! Of course if you have a slight carry-over, or something your chromatographic software identifies as an IS peak, the software will calculate a concentration. But it is meaningless and it should be disregarded. All you can do with this sample is check that you have no interference at the retention time of your IS, and compare the analyte peak area to that of the first calibration sample.
Regards
Ohlbe
❝ If a predose (without IS) shows a very high concentaration
You can't calculate a concentration in a sample without IS, by comparison to a standard curve with IS ! Of course if you have a slight carry-over, or something your chromatographic software identifies as an IS peak, the software will calculate a concentration. But it is meaningless and it should be disregarded. All you can do with this sample is check that you have no interference at the retention time of your IS, and compare the analyte peak area to that of the first calibration sample.
Regards
Ohlbe
Complete thread:
- Predose Conc BEAZ 2008-11-12 06:47
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- Predose Conc kaushal09 2008-11-12 11:31
- Predose ConcOhlbe 2008-11-12 12:00
- Predose Conc Geokad 2008-11-12 16:51
- Predose Conc srithan 2008-12-06 11:49
Ing. Helmut Schütz