Bioequivalence and Bioavailability Forum • Internal Standard Variation

Internal Standard Variation [Bioanalytics]

posted by Helmut – Vienna, Austria, 2008-08-15 19:29 (6519 d 19:46 ago) – Posting: # 2193
Views: 16,669

Servus, ElMaestro!

❝ It never hurts to have a SOP.

Yeah!

❝ Oder was?

I don’ know either; nice suggestions of yours (BTW - for our non-German speaking friends: “Oder was?” means “Or what?”).

❝ OK: This would work if the IS response follows a unimodal trend. But might not work if the IS-response has one or more extrema.

Ha! Of course we even can start some kind of control-chart business, but what for? QCs should be spread within the batch; if the batch passes, so what? An IS protects against the need of exact volume control during sample preparation – if half of the volume is spilled in one step, the analyte/IS ratio should stay the same (OK, as Ohlbe already pointed out last year, problems may arise close to the LLOQ).
I must confess, that I don’t get the point of the IS response variation discussion. :ponder:

—
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Helmut Schütz

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Complete thread:

Internal Standard Variation Dr. Harish L. Rao 2007-09-08 14:16
- Internal Standard Variation Helmut 2007-09-08 16:43
  - Internal Standard Variation Ohlbe 2007-09-08 22:32
    - Internal Standard Variation Helmut 2007-09-09 00:37
      - Internal Standard Variation Charl 2007-09-09 12:25
        
        Internal Standard Variation Jaime_R 2007-09-09 13:13
        
        IS vs ES methods Helmut 2007-09-09 15:05
        Internal Standard Variation Charl 2007-09-10 10:10
        
        Internal Standard Variation Jaime_R 2007-09-10 18:58
      - Internal Standard Variation Ohlbe 2007-09-09 23:20
        
        Internal Standard Variation Helmut 2007-09-10 20:33
- Internal Standard Variation vamshi 2008-08-14 12:33
  - Internal Standard Variation ElMaestro 2008-08-14 14:09
    - Internal Standard Variation Helmut 2008-08-14 20:28
      - Internal Standard Variation ElMaestro 2008-08-15 16:51
        
        Internal Standard VariationHelmut 2008-08-15 17:29