Bioequivalence and Bioavailability Forum

Think you for your explanations, for the first question i meant that actually i calculate my LIQ basing on formula of 5% of lower Cmax observed in bibliography i try to go lower than that on my HPLC to cover residual concentrations if observed later, we haven't yet a pharmacologue in our CRO (we are just team of scientists working on amoxicilline bioéquivalence), we have been taught that there is a formul to calculate LIQ from AUC ?

2/My question about concentration of internal standard: actually i use cefadroxil as described in articles as IS most recomended, in articles they describe different concentrations, i nedd to know how can we fix exactly this concentration , is it in relation with analyte concentration wich is amoxicilline at ULOQ or i have to test different concentrations of IS then analyte/IS later confused: ?



i hope receiving responses frome other member of forum, i dont know HOW !