Interference from Deuterated IS [Bioanalytics]

posted by Ohlbe – France, 2019-01-10 23:36 (2701 d 12:54 ago) – Posting: # 19769
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Dear VKB2207,

❝ You can probably evaluate the % contribution from IS at RT of drug


Sure. It's called the intercept :-D

❝ and then apply this factor in CC, QC nominal concentrations.


Certainly not. It is taken into consideration when calculating the calibration curve parameters, unless you are forcing the intercept through 0 (which is not to be done in bioanalysis).

❝ Hence measured concentrations would be inaccurate.


Not necessarily at high level of concentrations. Could indeed be problematic closer to the LLOQ.
—
Regards
Ohlbe

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