Bioequivalence and Bioavailability Forum

Spiking Solution Check [Bioanalytics]

posted by Jaime_R – Barcelona, 2008-05-20 16:16 (6606 d 15:55 ago) – Posting: # 1858
Views: 3,202

Hi Vishal!

❝ I have observed that Spiking Solution concentration (Vial Preparation)

❝ is prepared almost double to the nominal concentration.

Do I miss something?

Your 1^st dilution is 1:50 (200 uL -> 10 mL), making 2.024 ug/mL -> 40.48 ng/mL,
your 2^nd dilution is 1:25 (40 uL -> 1 mL), therefore 2.024 ug/mL -> 80.96 ng/mL.

Complete thread:

• Spiking Solution Check vish14184 2008-05-20 09:16 
  • Spiking Solution CheckJaime_R 2008-05-20 14:16
    • Spiking Solution Check vish14184 2008-05-20 15:03