Bioequivalence and Bioavailability Forum

Dear Samar Sameh,

❝ We are dealing with 2 metabolites both on the +ve ionization mode; the N-oxide (potency=96.8%) and sulfoxide (potency=99.9%)

I have no experience with sulfoxide. N-oxides are known for in-source dissociation. My understanding is that source temperature is an important factor. However if the metabolite is chromatographically separated from the parent, in-source dissociation should not create any problem.

If you are dealing with 2 metabolites and are finding potential issues, I would suggest to study the two separately in order to facilitate troubleshooting.