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RegisterMetabolite back conversion [Bioanalytics]
Hello Every body:
can any one please share with me the procedure conducted for the back conversion test during bioanalytical method validation. what we do is to spike the LLOQ samples of the drug with the maximum expected concentration of the metabolite, and the calculating the accuracy of the LLOQ through a calibration curve of the drug the covers a range from LLOQ to ULOQ. every time we have a failed accuracy, i dont know if there is really a back conversion or if our procedure is misleading.
BR
S.S
can any one please share with me the procedure conducted for the back conversion test during bioanalytical method validation. what we do is to spike the LLOQ samples of the drug with the maximum expected concentration of the metabolite, and the calculating the accuracy of the LLOQ through a calibration curve of the drug the covers a range from LLOQ to ULOQ. every time we have a failed accuracy, i dont know if there is really a back conversion or if our procedure is misleading.
BR
S.S
Complete thread:
- Metabolite back conversionsamar sameh 2017-07-16 07:47
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- Metabolite back conversion Ohlbe 2017-07-17 10:24
- Metabolite back conversion samar sameh 2017-07-18 13:29
- Metabolite back conversion Ohlbe 2017-07-18 16:29
- Metabolite back conversion samar sameh 2017-07-18 13:29
- Metabolite back conversion Ohlbe 2017-07-17 10:24
Ing. Helmut Schütz