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Dear Roshini,
It is always a good practice to take plasma sample first into the vial and add internal standard to it. But your practical concern of missing of internal standard (IS) addition to some vials, if IS is added later, is valid. Hence, it is better to practically prove (as you said, as part of your Robustness experiments) that it makes no significant difference whether you add 'IS to plasma' or 'plasma to IS'. In addition, you need to state in your method protocol or method SOP, what you planned to do. For example, you may say, "take so and so volume of plasma sample into the vials containing so and so IS".
With best regards,
V.Pardhasaradhi
It is always a good practice to take plasma sample first into the vial and add internal standard to it. But your practical concern of missing of internal standard (IS) addition to some vials, if IS is added later, is valid. Hence, it is better to practically prove (as you said, as part of your Robustness experiments) that it makes no significant difference whether you add 'IS to plasma' or 'plasma to IS'. In addition, you need to state in your method protocol or method SOP, what you planned to do. For example, you may say, "take so and so volume of plasma sample into the vials containing so and so IS".
With best regards,
V.Pardhasaradhi
Complete thread:
- Addition of Internal Standard Roshini 2008-01-21 12:36
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- Addition of Internal Standardvpardhasaradhi 2008-01-25 04:39
- Rubustness experiments Helmut 2008-01-26 13:21
- Addition of Internal Standardvpardhasaradhi 2008-01-25 04:39
Ing. Helmut Schütz