Bioequivalence and Bioavailability Forum

Spiking volume-total volume [Bioanalytics]

posted by Ladi – Thailand, 2015-09-30 16:55 (3525 d 04:43 ago) – Posting: # 15501
Views: 7,196

Dear ElMaestro,

❝ My own chemistry teacher had to take early retirement because she was so perplexed by my persistent miscalculations.

❝ You have xyz ng analyte per 20 ul; you then dilute that entire volume to 500 ul before execution. Now your conc. is xyz/25 units ng/ul (or xyz/(500/20)).

❝ And you do at this point not possess a solution which is xyz/(480/20).

I completely agree with you.

But people are really questioning this because the volume of 'study sample' use is only 480 uL. So they are curious if the total plasma used put in report should be 480 uL or 500 uL. And I am trying to figure out how to explain it.

Still arguing in the lab ,
Ladi

Complete thread:

• spiked samples preparation Ladi 2015-09-17 07:04 
  • Spiking volume Ohlbe 2015-09-17 10:45
    • Spiking volume Ladi 2015-09-17 13:02
    • Spiking volume-total volume Ladi 2015-09-30 13:42
      • Spiking volume-total volume ElMaestro 2015-09-30 13:57
        • Spiking volume-total volumeLadi 2015-09-30 14:55