Bioequivalence and Bioavailability Forum

Dear Sir,

I have some query regarding the definiation of dead volume which is given below,

Some of the reference books gives the definition of dead volume as follows,

"Time required by the inert sample to migrate from the column inlet to column outlet without 'being retained' non as a dead volume"

now my question is,

In column specification we have a calcuation for capacity facotor K' (formula is k'=tr-to/to) and as a 'to', first retained peak of the combination is consider. kindly give me the actul procedure to calculate the dead volume and also discribe how it is effect my chromatogram if the value of k' is >10, < 1 and >30 ?

Regards,

Vishal Vayeda