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Dear HS,
100 % agree. My answer was much too short there, and metabolites add more risks of interferences. But this is not specific to multiple co-administered drugs and it can happen with just one analyte. My worry is not really with UV or fluo detection, rather with MS. Many labs just forget that LC/MS/MS starts with LC and go for very short run times with a reduced sample preparation. If you don't separate your metabolite chromatographically you may end with real trouble, such as in the case of in-source dissociation of acyl-glucuronides or N-oxydes. And the interference in that case will be with the parent compound itself, not with a co-administered drug.
Regards
Ohlbe
❝ Your suggestions are quite reasonable, but metabolites may make the life of the analyst quite miserable...
100 % agree. My answer was much too short there, and metabolites add more risks of interferences. But this is not specific to multiple co-administered drugs and it can happen with just one analyte. My worry is not really with UV or fluo detection, rather with MS. Many labs just forget that LC/MS/MS starts with LC and go for very short run times with a reduced sample preparation. If you don't separate your metabolite chromatographically you may end with real trouble, such as in the case of in-source dissociation of acyl-glucuronides or N-oxydes. And the interference in that case will be with the parent compound itself, not with a co-administered drug.
Regards
Ohlbe
Complete thread:
- Method Validation chiragkhatri 2007-12-14 06:21
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- Method Validation Ohlbe 2007-12-15 12:05
- Method Validation chiragkhatri 2007-12-15 12:22
- Metabolites! Helmut 2007-12-15 13:42
- Metabolites!Ohlbe 2007-12-16 13:32
- Metabolites! Helmut 2007-12-16 13:50
- Metabolites!Ohlbe 2007-12-16 13:32
- Method Validation Ohlbe 2007-12-15 12:05
Ing. Helmut Schütz