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RegisterMethod Validation [Bioanalytics]
Dear Chirag,
No guideline jumps to my mind on this topic. What I would suggest would be to prepare and analyse 3 different QC samples, each spiked at a low concentration (LLOQ or LQC) with one analyte (1, 2 or 3) and ULOQ with the other two. If there is any interference (either directly visible, or influencing the ionisation of your analyte or IS if you are using MS/MS) you would have maximum chances to see an impact on the accuracy.
Regards
Ohlbe
No guideline jumps to my mind on this topic. What I would suggest would be to prepare and analyse 3 different QC samples, each spiked at a low concentration (LLOQ or LQC) with one analyte (1, 2 or 3) and ULOQ with the other two. If there is any interference (either directly visible, or influencing the ionisation of your analyte or IS if you are using MS/MS) you would have maximum chances to see an impact on the accuracy.
Regards
Ohlbe
Complete thread:
- Method Validation chiragkhatri 2007-12-14 06:21
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- Method ValidationOhlbe 2007-12-15 12:05
- Method Validation chiragkhatri 2007-12-15 12:22
- Metabolites! Helmut 2007-12-15 13:42
- Metabolites! Ohlbe 2007-12-16 13:32
- Metabolites! Helmut 2007-12-16 13:50
- Metabolites! Ohlbe 2007-12-16 13:32
- Method ValidationOhlbe 2007-12-15 12:05
Ing. Helmut Schütz