Bioequivalence and Bioavailability Forum

Dear members,

I have a combination of Drug 1+2+3. Since it is not possible to analyze all three together, I have validated method for drug 1 separately and drug 2and 3 will be validated in combination.

The point here is since subject samples will be in combination of all three, I will be analyzing it twice with two different methods, but the validation is not done in presence of drug1 for drug2 and drug3 and vice versa.

I nedd suggestions as to how to plan an experiment so that it can be proved that the method used for Drug1 does not have any interference of drug 2 and drug3 and vice-versa.

Any reference to guidelines are welcome.

Thanks in advance

Chirag Khatri