Bioequivalence and Bioavailability Forum • Recovery

Recovery [Bioanalytics]

posted by nobody – 2014-03-23 12:01 (4482 d 06:23 ago) – Posting: # 12689
Views: 6,613

❝ What about samples that do not go through extraction step ? Plasma samples treated with just the addition of ACN or methanol before injecting into LCMSMS.

❝ We have been doing recovery for sample treated in this way. Is it necessary to have recovery ?

You might precipitate in real samples some of the analyte with the protein in your sample when adding ACN or methanol. But in general, as long as you treat standards, QCs and verum samples identically (and have representative empty matrix), in most cases recovery is not very interesting. More a parameter to take care for while debugging, in my opinion...

Kind regards

—
Kindest regards, nobody

Complete thread:

Recovery sarada06884 2014-03-20 09:55
- Recovery Ohlbe 2014-03-20 11:19
  - Recovery Ken Peh 2014-03-23 07:23
    - Recoverynobody 2014-03-23 11:01
      - Recovery Ohlbe 2014-03-23 16:28
    - Recovery Ohlbe 2014-03-23 16:37
      - Recovery Ken Peh 2014-03-27 19:16