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Registermatrix effect # extraction recovery [Bioanalytics]
posted by Helmut 
– Vienna, Austria, 2007-10-17 13:03 (6829 d 06:00 ago) – Posting: # 1208
Views: 6,815
Dear Charl
The matrix effect (mainly in LC/MS-MS, but to a minor extent in fluorescence [quenching]) has nothing to do with recovery. It’s possible to get just 2% of response after sample preparation (for a method with 100% recovery) compared to a matrix-free dilution due to ion-suppression.
❝ extraction could be performed with high efficiency, some times you cant get 100% recovery, does that mean you have ion effect?
❝ or is it as simple as the extraction capacity is not high enough for 100% recovery !
The matrix effect (mainly in LC/MS-MS, but to a minor extent in fluorescence [quenching]) has nothing to do with recovery. It’s possible to get just 2% of response after sample preparation (for a method with 100% recovery) compared to a matrix-free dilution due to ion-suppression.
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Helmut Schütz
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Dif-tor heh smusma 🖖🏼 Довге життя Україна!
Helmut Schütz
The quality of responses received is directly proportional to the quality of the question asked. 🚮
Science Quotes
Complete thread:
- matrix effect for protein precipitation pb070681 2007-10-16 08:26
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- matrix effect for protein precipitation Helmut 2007-10-16 13:38
- matrix effect for protein precipitation Charl 2007-10-17 09:04
- matrix effect # extraction recoveryHelmut 2007-10-17 11:03
- matrix effect # extraction recovery vpardhasaradhi 2007-10-18 12:48
- matrix effect for protein precipitation Charl 2007-10-17 09:04
- matrix effect for protein precipitation Helmut 2007-10-16 13:38
Ing. Helmut Schütz