Bioequivalence and Bioavailability Forum

❝ Agreed. And I think this volume could easily be further reduced. Many labs still develop methods starting with 500 µl of plasma, just like in the old days of HPLC/UV, even though they are now using LC-MS/MS which can easily achieve an LLOQ several orders of magnitude lower. It would be perfectly feasible in most cases to only use 100 µl of plasma and reduce the blood sampling volume to 3 ml. This would still leave more than enough for repeat analysis and ISR.

IIRC there are practical limitations WRT to obtaining plasma (size of Monvette, centrifugation, hemolysis, pipetting/storage of plasma?) so: not so easy to downsize in practice, I think. Might depend strongly on CRO, I guess...