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RegisterCentrifugation temperature [Regulatives / Guidelines]
Dear Ken,
I think the issue is not just degradation in plasma but in whole blood. I would expect the lower temperature to decrease the activity of the various erythrocyte enzymes. As the whole blood stability is usually not studied, most labs prefer to spin in a refrigerated centrifuge to limit the risk of degradation of the analyte and metabolites.
Anyway, I agree with the previous posts: there is no temperature specified in the FDA and EMA guidelines (I don't know about others), just use whatever works / is needed.
regards
Ohlbe
❝ So far, we do not face any problems of drug degradation in plasma.
I think the issue is not just degradation in plasma but in whole blood. I would expect the lower temperature to decrease the activity of the various erythrocyte enzymes. As the whole blood stability is usually not studied, most labs prefer to spin in a refrigerated centrifuge to limit the risk of degradation of the analyte and metabolites.
Anyway, I agree with the previous posts: there is no temperature specified in the FDA and EMA guidelines (I don't know about others), just use whatever works / is needed.
regards
Ohlbe
—
Regards
Ohlbe
Regards
Ohlbe
Complete thread:
- Centrifugation Timings Irukulla Shravan Kumar 2013-03-18 07:02
![[Mix]](https://static.bebac.at/img/mix.png "open in Mix view")
- Centrifugation temperature ElMaestro 2013-03-18 14:51
- Centrifugation temperature Helmut 2013-03-18 16:46
- Centrifugation temperature Ken Peh 2013-03-18 17:45
- Centrifugation temperatureOhlbe 2013-03-18 18:44
- rpm vs. ×g Helmut 2013-03-18 23:14
- rpm vs. ×g Irukulla Shravan Kumar 2013-03-19 05:00
- Centrifugation temperature Ken Peh 2013-03-18 17:45
- Centrifugation temperature Helmut 2013-03-18 16:46
- Centrifugation temperature ElMaestro 2013-03-18 14:51
Ing. Helmut Schütz