Bioequivalence and Bioavailability Forum

Hello bharahti,

❝ We are dealing with one of the endogenous compound. LOQ is high nearly 1 ng/mL where as Cmax itself is around 8-9ng/mL

❝ One of the lab offered a method with 0.25 ng/mL with some interferences.

❝ Is this acceptable (i.e with interferences)? If it is acceptable, do we need to do any corrections?

Before I try to guess wildly at this I'd like to hear what the normal endogenous range is (min, max). Could you comment?

❝ Does using internal standard help to any extent?

Is this done by chromatography? If so then I think it is almost unusual if there's no internal standard.