Bioequivalence and Bioavailability Forum

Hello Members,

We have carried out in-vitro binding study at 4.0 and 7.0 pH with and without acid pretreatment. Here, we have noticed that at pH 7.0 with and without pre-treatment could obtain promising results, whereas at pH 4.0 with and without pre-treatment its not.

My questions are:

1. What is the reason for this cause?

2. Will pH difference leads the bad results or any experiment error may involved?

3. Any reported peer reviewed paper on this matter?


Thanks,