nvs ☆ India, 2007-05-21 14:32 (6178 d 05:48 ago) Posting: # 734 Views: 4,859 |
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Dear all, can any one let me know on what is the main differentiation between selectivity and matrix effect,and main purpose to conduct matrix effect in validation. |
Helmut ★★★ Vienna, Austria, 2007-05-21 22:44 (6177 d 21:36 ago) @ nvs Posting: # 735 Views: 4,001 |
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Dear nvs! ❝ can any one let me know on what is the main differentiation between ❝ selectivity and matrix effect,and main purpose to conduct matrix effect in ❝ validation.
The opposite is the case in LC/MS-MS, where large amounts of interfering compounds may co-elute with you analyte without being even noticed. Unfortunatelly these compounds may alter (suppress or enhance) the ionization (and therefore the signal) of the analyte. Making things even worse, not only the current chromatogram may be influenced, but the sensitivity of subsequent ones may also gradually be influenced by some kind of 'memory' of the ion-source. The lesson we have learned the hard way in the last years was this one: Contrary to the promises of vendors of MS-instruments (protein precipitation and inject), although LC/MS-MS is a powerful and very selective analytical technique, sample clean-up is even more important than in 'classical' HPLC/GC. — Dif-tor heh smusma 🖖🏼 Довге життя Україна! Helmut Schütz The quality of responses received is directly proportional to the quality of the question asked. 🚮 Science Quotes |