SanTo
☆    

Indonesia,
2019-05-03 04:55

Posting: # 20268
Views: 393
 

 Bioanalysis method of iso­sorbide mono­nitrate [Bioanalytics]

Hi,

We have tried to develop a bioanalysis method of isosorbide mononitrate but it has not been successful. When tuning with LC MSMS, the parent ion intensity is e7 while the intensity of the daughter ion is e5. What we have done is with negative ESI at m / z 249.92> 59, with column C18, the mobile phase used is methanol and ammonium acetate.
Our target is 3.5 ng / mL in human plasma but when injection of samples in the solvent (not extracted) the area is not present.

Please help solve our problem. Thanks.
cakhatri
★    

India,
2019-05-28 11:13

@ SanTo
Posting: # 20303
Views: 173
 

 Bioanalysis method of iso­sorbide mono­nitrate

Dear SanTo,

The molecular weight seems to be 191.139 g/mol. How did you get 249.92 for parent compound.

Regards
Chirag
Activity
 Thread view
Bioequivalence and Bioavailability Forum |  Admin contact
19,514 posts in 4,141 threads, 1,336 registered users;
online 8 (0 registered, 8 guests [including 6 identified bots]).
Forum time (Europe/Vienna): 21:05 CEST

I have not failed 700 times. I have not failed once.
I have succeeded in proving
that those 700 ways will not work.    Thomas Alva Edison

The BIOEQUIVALENCE / BIOAVAILABILITY FORUM is hosted by
BEBAC Ing. Helmut Schütz
HTML5