Simultaneous analytical method [Bioanalytics]

posted by Ohlbe – France, 2018-09-13 16:06 (2023 d 07:34 ago) – Posting: # 19287
Views: 3,658

Dear giang nidqc

❝ Could it be a possibility that the absence of one APIs may affect the final concentration of other APIs (regarding sample preparation and peak response)?


I agree with Helmut's post above. There is just one thing I'm thinking of: how was the spiking of calibration and QC samples done when you used 5 analytes ? Did you use a single spiking solution containing all analytes (usual practice), or separate working solutions ? If you used a single, combined solution at each level concentration, no problem. If you used separate spiking solutions, you will have a different proportion of solvent added to the plasma if you have less analytes, which may influence your recovery and matrix effects.

Regards
Ohlbe

Complete thread:

UA Flag
Activity
 Admin contact
22,957 posts in 4,819 threads, 1,636 registered users;
77 visitors (0 registered, 77 guests [including 4 identified bots]).
Forum time: 22:40 CET (Europe/Vienna)

Nothing shows a lack of mathematical education more
than an overly precise calculation.    Carl Friedrich Gauß

The Bioequivalence and Bioavailability Forum is hosted by
BEBAC Ing. Helmut Schütz
HTML5