Bioequivalence and Bioavailability Forum

Dear Mr. NVS,

Retention times in LC-MS/MS analysis are important with respect to separation of analyte(s) and the internal standard from the other impurities eluting out from the analytical column. Unfortunately, the elution of these impurities can not be seen or noticed and these may cause ion suppression or enhancement. While working at very lower concentrations of the analyte where the internal standard concentration is comparatively high, even the internal standard can influence (either suppress or enhance) the ionization of the analyte.

Bad peak shapes need not be because of interferences. Bad peak shapes can also be because of unsuitable chromatographgic conditions (analytical column or mobile phase).

Hence, it is important to check the matrix effect (ion suppression or enhancement) and improve the chromatographic conditions for better peak shapes and less matrix effects.

V.Pardhasaradhi

--
Edit: Full quote removed. Please see this post! [HS]