sample centrifugation [Study Performance]
Hi ssk!
g: approximately 9.81 m/s².
It's good practice to state something like '1500 × g' (1500times acceleration due to earth's gravity) in order to be able to compare different centrifuges.
Stating rpm alone means nothing (since the diameter of the rotor is not given).
For a conversion have a look at this document or this nomogram.
Cooled
Actually you don't know the temperature of the sample, but it's quite common to set the temperature of the centrifuge to +4 °C.
As already stated it should allow for easy separation of plasma.
I don't believe that sponsors do calculate anything - mainly they are skillful in 'copy-and-paste'...
❝ What does the g stand for.
g: approximately 9.81 m/s².
It's good practice to state something like '1500 × g' (1500times acceleration due to earth's gravity) in order to be able to compare different centrifuges.
Stating rpm alone means nothing (since the diameter of the rotor is not given).
For a conversion have a look at this document or this nomogram.
❝ What is the standard temperature at which the centrifugation usually done.
Cooled

Actually you don't know the temperature of the sample, but it's quite common to set the temperature of the centrifuge to +4 °C.
❝ How are these parameters calculated by the sponsor (rpm,temperature and the duration).
As already stated it should allow for easy separation of plasma.
I don't believe that sponsors do calculate anything - mainly they are skillful in 'copy-and-paste'...

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Regards, Jaime
Regards, Jaime
Complete thread:
- sample centrifugation ssk 2006-10-12 11:20
- sample centrifugation Jaime_R 2006-10-12 12:04
- sample centrifugation ssk 2006-10-12 12:29
- sample centrifugationJaime_R 2006-10-12 13:15
- sample centrifugation ssk 2006-10-12 12:29
- sample centrifugation Jaime_R 2006-10-12 12:04